111 research outputs found
Personnel Airdrop Risk Assessment Using Bootstrap Sampling
Previous work on personnel airdrop problems involving jumpers has been (1) event-oriented entanglement rates, (2) number of canopy bumps, (3) landing injuries, and (4) deaths. The thesis expands this area of research by developing cumulative distribution functions of maximum possible chute entanglement risk for the C-17 using bootstrap techniques. By comparing the effects of various C-17 aircraft configurations on the entanglement CDF, this thesis shows that under certain configurations the risk of centerline entanglement for the C-17 is less than for the C-141
Genetic Representations for Evolutionary Minimization of Network Coding Resources
We demonstrate how a genetic algorithm solves the problem of minimizing the
resources used for network coding, subject to a throughput constraint, in a
multicast scenario. A genetic algorithm avoids the computational complexity
that makes the problem NP-hard and, for our experiments, greatly improves on
sub-optimal solutions of established methods. We compare two different genotype
encodings, which tradeoff search space size with fitness landscape, as well as
the associated genetic operators. Our finding favors a smaller encoding despite
its fewer intermediate solutions and demonstrates the impact of the modularity
enforced by genetic operators on the performance of the algorithm.Comment: 10 pages, 3 figures, accepted to the 4th European Workshop on the
Application of Nature-Inspired Techniques to Telecommunication Networks and
Other Connected Systems (EvoCOMNET 2007
Evolutionary Approaches to Minimizing Network Coding Resources
We wish to minimize the resources used for network coding while achieving the
desired throughput in a multicast scenario. We employ evolutionary approaches,
based on a genetic algorithm, that avoid the computational complexity that
makes the problem NP-hard. Our experiments show great improvements over the
sub-optimal solutions of prior methods. Our new algorithms improve over our
previously proposed algorithm in three ways. First, whereas the previous
algorithm can be applied only to acyclic networks, our new method works also
with networks with cycles. Second, we enrich the set of components used in the
genetic algorithm, which improves the performance. Third, we develop a novel
distributed framework. Combining distributed random network coding with our
distributed optimization yields a network coding protocol where the resources
used for coding are optimized in the setup phase by running our evolutionary
algorithm at each node of the network. We demonstrate the effectiveness of our
approach by carrying out simulations on a number of different sets of network
topologies.Comment: 9 pages, 6 figures, accepted to the 26th Annual IEEE Conference on
Computer Communications (INFOCOM 2007
Flow Characteristics Around Step-Up Street Canyons with Various Building Aspect Ratios
We investigate the flow characteristics around step-up street canyons with various building aspect ratios (ratio of along-canyon building length to street-canyon width, and upwind building height to downwind building height) using a computational fluid dynamics (CFD) model. Simulated results are validated against experimental wind-tunnel results, with the CFD simulations conducted under the same building configurations as those in the wind-tunnel experiments. The CFD model reproduces the measured in-canyon vortex, rooftop recirculation zone above the downwind building, and stagnation point position reasonably well. We analyze the flow characteristics, focusing on the structural change of the in-canyon flows and the interaction between the in- and around-canyon flows with the increase of building-length ratio. The in-canyon flows undergo development and mature stages as the building-length ratio increases. In the development stage (i.e., small building-length ratios), the position of the primary vortex wanders, and the incoming flow closely follows both the upstream and downstream building sidewalls. As a result, increasing momentum transfer from the upper layer contributes to a momentum increase in the in-canyon region, and the vorticity in the in-canyon region also increases. In the mature stage (i.e., large building-length ratios), the primary vortex stabilizes in position, and the incoming flow no longer follows the building sidewalls. This causes momentum loss through the street-canyon lateral boundaries. As the building-length ratio increases, momentum transfer from the upper layer slightly decreases, and the reverse flow, updraft, and streamwise flow in the in-canyon region also slightly decrease, resulting in vorticity reduction
Selective phase transformation of layered double hydroxides into mixed metal oxides for catalytic CO oxidation
Phase transformation from layered double hydroxides (LDHs) into mixed metal oxides (MMOs) has been widely used in various catalytic applications owing to its numerous advantages over conventional synthesis methods. Herein we report the results of selective phase transformation of LDHs into spinels and delafossites for the preparation of phase-pure MMO catalysts. Pure cuprous delafossites and cupric spinels were selectively obtained through heat treatment of Cu-based LDHs followed by post-treatments. This enabled the study of the crystalline-phase-dependent CO oxidation activity of the MMO catalysts and their physicochemical properties. The spinel catalysts exhibited higher CO oxidation activities, in comparison with those of the delafossites, with greater redox properties and improved active sites for CO adsorption. Although the crystalline phases were derived from the same LDH precursors, the catalytic properties of the end product were greatly influenced by their crystal structures
Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution
Background
Methane, a main component of natural gas and biogas, has gained much attention as an abundant and low-cost carbon source. Methanotrophs, which can use methane as a sole carbon and energy source, are promising hosts to produce value-added chemicals from methane, but their metabolic engineering is still challenging. In previous attempts to produce lactic acid (LA) from methane, LA production levels were limited in part due to LA toxicity. We solved this problem by generating an LA-tolerant strain, which also contributes to understanding novel LA tolerance mechanisms.
Results
In this study, we engineered a methanotroph strain Methylomonas sp. DH-1 to produce d-lactic acid (d-LA) from methane. LA toxicity is one of the limiting factors for high-level production of LA. Therefore, we first performed adaptive laboratory evolution of Methylomonas sp. DH-1, generating an LA-tolerant strain JHM80. Genome sequencing of JHM80 revealed the causal gene watR, encoding a LysR-type transcription factor, whose overexpression due to a 2-bp (TT) deletion in the promoter region is partly responsible for the LA tolerance of JHM80. Overexpression of the watR gene in wild-type strain also led to an increase in LA tolerance. When d form-specific lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 was introduced into the genome while deleting the glgA gene encoding glycogen synthase, JHM80 produced about 7.5-fold higher level of d-LA from methane than wild type, suggesting that LA tolerance is a critical limiting factor for LA production in this host. d-LA production was further enhanced by optimization of the medium, resulting in a titer of 1.19 g/L and a yield of 0.245 g/g CH4.
Conclusions
JHM80, an LA-tolerant strain of Methylomonas sp. DH-1, generated by adaptive laboratory evolution was effective in LA production from methane. Characterization of the mutated genes in JHM80 revealed that overexpression of the watR gene, encoding a LysR-type transcription factor, is responsible for LA tolerance. By introducing a heterologous lactate dehydrogenase gene into the genome of JHM80 strain while deleting the glgA gene, high d-LA production titer and yield were achieved from methane.This work was supported by C1 Gas Refnery Program through the National Research Foundation of Korean (NRF) funded by the Ministry of Science and ICT (2016M3D3A01913245)
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